Difference between revisions of "Team:NCHU Taichung/Parts"

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{{NCHU_Taichung}}
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<groupparts class="ui celled table">iGEM18 NCHU_Taichung</groupparts>
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<div class="ui main text container">
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    <div class="ui segments" id="mainbox">
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      <div class="ui raised segment" id="mainbox">
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        <h1 class="ui hidden header"></h1>
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        <div class="ui huge header" id="top">Parts Overview</div>
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        <h2 class="ui header">Parts</h2>
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        <p class="longP">Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase
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          DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part
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          of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene
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          and replace the ori and also the resistance gene, which can also be seen in the basic part. </p>
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        <h3 class="ui dividing header">Basic Parts</h3>
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        <div class="ui bulleted list">
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
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            halogenated compounds</div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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            of pMP4657 and BBa_J364007 in pSB1C3. </div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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            of pMP4657 and BBa_J364007 in pSB1C3. </div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
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            Ori of pBBR1 and BBa_J364007 in pSB1C3. </div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
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            carrier, siderophore, which is similar in structure with dioxin.</div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.</div>
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        </div>
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        <h3 class="ui dividing header">Intermediate Parts</h3>
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        <div class="ui bulleted list">
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
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            site.</div>
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        </div>
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        <h3 class="ui dividing header">Composite Parts</h3>
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        <div class="ui bulleted list">
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.</div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
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            let three gene be expressed in one circuit.</div>
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          <div class="item">
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            <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.</div>
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        </div>
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        <h1 class="ui hidden header"></h1>
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        <table class="ui selectable teal inverted celled small table">
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          <thead>
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            <tr>
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              <th class="three wide">Name</th>
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              <th class="three wide">Type</th>
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              <th class="four wide">Description</th>
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              <th class="three wide">Designer</th>
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              <th class="three wide">Length</th>
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            </tr>
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          </thead>
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          <tbody></tbody>
 +
          <tr>
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            <td class="selectable">
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              <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000 </a>
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            </td>
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            <td>Coding </td>
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            <td>dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf </td>
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            <td>Tsu-Wang Sun </td>
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            <td> </td>
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          </tr>
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          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001 </a>
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            </td>
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            <td>Coding </td>
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            <td>2-haloacid dehalogenase(HAD) </td>
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            <td>Tsu-Wang Sun </td>
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            <td>780</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002 </a>
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            </td>
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            <td>Coding </td>
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            <td>2-haloacid dehalogenase plus laccase with robosome binding site </td>
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            <td>Tsu-Wang Sun </td>
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            <td>2408</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003 </a>
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            </td>
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            <td>Coding </td>
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            <td>p+HL </td>
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            <td>Tsu-Wang Sun </td>
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            <td>6</td>
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          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004 </a>
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            </td>
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            <td>Coding </td>
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            <td>pME60C shuttle vector </td>
 +
            <td>Tsu-Wang Sun </td>
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            <td>6489</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005 </a>
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            </td>
 +
            <td>Coding </td>
 +
            <td>2-haloacid dehalogenase plus laccase with promoter and ribosome binding site </td>
 +
            <td>Tsu-Wang Sun </td>
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            <td>2451</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006 </a>
 +
            </td>
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            <td>Coding </td>
 +
            <td>TonB + 2-haloacid dehalogenase + laccase </td>
 +
            <td>Tsu-Wang Sun </td>
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            <td>4721</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007 </a>
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            </td>
 +
            <td>Plasmid_Backbone </td>
 +
            <td>cm 4657 </td>
 +
            <td>Tsu-Wang Sun </td>
 +
            <td>770</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008 </a>
 +
            </td>
 +
            <td>Plasmid_Backbone </td>
 +
            <td>pME60C2 shuttle vector </td>
 +
            <td>Tsu-Wang Sun </td>
 +
            <td>5491</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009 </a>
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            </td>
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            <td>Plasmid_Backbone </td>
 +
            <td>kan bbrf2 </td>
 +
            <td>Tsu-Wang Sun </td>
 +
            <td>4218</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010 </a>
 +
            </td>
 +
            <td>Coding </td>
 +
            <td>TonB-dependent receptor (TonB) </td>
 +
            <td>Tsu-Wang Sun </td>
 +
            <td>2244</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011 </a>
 +
            </td>
 +
            <td>Coding </td>
 +
            <td>Laccase (TLcc) </td>
 +
            <td>Tsu-Wang Sun </td>
 +
            <td>1584</td>
 +
          </tr>
 +
          <tr>
 +
            <td class="selectable">
 +
              <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012 </a>
 +
            </td>
 +
            <td>Coding </td>
 +
            <td>TonB with promoter and ribosome binding site </td>
 +
            <td>Yu-Hsi Lin </td>
 +
            <td>2305 </td>
 +
          </tr>
 +
        </table>
 +
      </div>
 +
    </div>
 +
  </div>
 
{{NCHU_Taichung/footer}}
 
{{NCHU_Taichung/footer}}

Revision as of 18:35, 17 October 2018

NCHU_Taichung

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Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

           <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
halogenated compounds
           <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
of pMP4657 and BBa_J364007 in pSB1C3. 
           <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
of pMP4657 and BBa_J364007 in pSB1C3. 
           <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
Ori of pBBR1 and BBa_J364007 in pSB1C3. 
           <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
carrier, siderophore, which is similar in structure with dioxin.
<a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

           <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
site.

Composite Parts

<a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.
           <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
let three gene be expressed in one circuit.
<a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.

<thead> </thead> <tbody></tbody>
Name Type Description Designer Length 
             <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000 </a>
Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun 
             <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001 </a>
Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780 
             <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002 </a>
Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408 
             <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003 </a>
Coding p+HL Tsu-Wang Sun 6 
             <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004 </a>
Coding pME60C shuttle vector Tsu-Wang Sun 6489 
             <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005 </a>
Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451 
             <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006 </a>
Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721 
             <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007 </a>
Plasmid_Backbone cm 4657 Tsu-Wang Sun 770 
             <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008 </a>
Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491 
             <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009 </a>
Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218 
             <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010 </a>
Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244 
             <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011 </a>
Coding Laccase (TLcc) Tsu-Wang Sun 1584 
             <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012 </a>
Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305