Difference between revisions of "Team:NCHU Taichung/Parts"

Line 11: Line 11:
 
           and replace the ori and also the resistance gene, which can also be seen in the basic part. </p>
 
           and replace the ori and also the resistance gene, which can also be seen in the basic part. </p>
 
         <h3 class="ui dividing header">Basic Parts</h3>
 
         <h3 class="ui dividing header">Basic Parts</h3>
−
         <div class="ui bulleted list">
+
         <ol class="ui ordered list">
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
 
             <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
−
             halogenated compounds</div>
+
             halogenated compounds</li>
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
 
             <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
−
             of pMP4657 and BBa_J364007 in pSB1C3. </div>
+
             of pMP4657 and BBa_J364007 in pSB1C3. </li>
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
 
             <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
−
             of pMP4657 and BBa_J364007 in pSB1C3. </div>
+
             of pMP4657 and BBa_J364007 in pSB1C3. </li>
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
 
             <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
−
             Ori of pBBR1 and BBa_J364007 in pSB1C3. </div>
+
             Ori of pBBR1 and BBa_J364007 in pSB1C3. </li>
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
 
             <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
−
             carrier, siderophore, which is similar in structure with dioxin.</div>
+
             carrier, siderophore, which is similar in structure with dioxin.</li>
−
           <div class="item">
+
           <li value="・">
−
             <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.</div>
+
             <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.</li>
−
         </div>
+
         </ol>
 
         <h3 class="ui dividing header">Intermediate Parts</h3>
 
         <h3 class="ui dividing header">Intermediate Parts</h3>
−
         <div class="ui bulleted list">
+
         <ol class="ui ordered list">
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
 
             <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
−
             site.</div>
+
             site.</li>
−
         </div>
+
         </ol>
 
         <h3 class="ui dividing header">Composite Parts</h3>
 
         <h3 class="ui dividing header">Composite Parts</h3>
−
         <div class="ui bulleted list">
+
         <ol class="ui ordered list">
−
           <div class="item">
+
           <li value="・">
−
             <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.</div>
+
             <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.</li>
−
           <div class="item">
+
           <li value="・">
 
             <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
 
             <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
−
             let three gene be expressed in one circuit.</div>
+
             let three gene be expressed in one circuit.</li>
−
           <div class="item">
+
           <li value="・">
−
             <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.</div>
+
             <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.</li>
−
         </div>
+
         </ol>
 
         <h1 class="ui hidden header"></h1>
 
         <h1 class="ui hidden header"></h1>
 
         <table class="ui selectable teal inverted celled small table">
 
         <table class="ui selectable teal inverted celled small table">

Revision as of 18:41, 17 October 2018

NCHU_Taichung

Home
Project
Description Background Design Model Result Demonstrate
Parts
Overview Improve
Human Practice
Integrated Introduction Conference Engagement Entrepreneurship
Lab
Interlab Notebook Protocol Safety
Team
Members Attributions Collaborations
Medals Prizes

Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
  2. <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  3. <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  4. <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
  5. <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
  6. <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.
  2. <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
  3. <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.

<thead> </thead> <tbody></tbody>
Name Type Description Designer Length 
             <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000 </a>
Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun 
             <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001 </a>
Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780 
             <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002 </a>
Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408 
             <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003 </a>
Coding p+HL Tsu-Wang Sun 6 
             <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004 </a>
Coding pME60C shuttle vector Tsu-Wang Sun 6489 
             <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005 </a>
Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451 
             <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006 </a>
Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721 
             <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007 </a>
Plasmid_Backbone cm 4657 Tsu-Wang Sun 770 
             <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008 </a>
Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491 
             <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009 </a>
Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218 
             <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010 </a>
Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244 
             <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011 </a>
Coding Laccase (TLcc) Tsu-Wang Sun 1584 
             <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012 </a>
Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305