Difference between revisions of "Team:NCHU Taichung/Parts"
| Line 1: | Line 1: | ||
{{NCHU_Taichung/navbar}} | {{NCHU_Taichung/navbar}} | ||
| − | + | <div class="ui main text container"> | |
<div class="ui segments" id="mainbox"> | <div class="ui segments" id="mainbox"> | ||
<div class="ui raised segment" id="mainbox"> | <div class="ui raised segment" id="mainbox"> | ||
| Line 13: | Line 13: | ||
<ol class="ui ordered list"> | <ol class="ui ordered list"> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a> | + | <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001:</a> This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of |
| − | + | halogenated compounds</nowiki> | |
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a | + | <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004:</a> This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori |
| − | + | of pMP4657 and BBa_J364007 in pSB1C3. </nowiki> | |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a | + | <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007:</a> This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori |
| − | + | of pMP4657 and BBa_J364007 in pSB1C3. </nowiki> | |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a | + | <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008:</a> This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the |
| − | + | Ori of pBBR1 and BBa_J364007 in pSB1C3. </nowiki> | |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a | + | <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010:</a> This part is coding for TonB-dependent receptor, which can actively transport the ferric ion |
| − | + | carrier, siderophore, which is similar in structure with dioxin.</nowiki> | |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a | + | <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011:</a> This part is coding for Laccase, which is related to lignin degradation.</nowiki> |
| − | + | ||
</li> | </li> | ||
</ol> | </ol> | ||
| Line 44: | Line 39: | ||
<ol class="ui ordered list"> | <ol class="ui ordered list"> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a | + | <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002:</a> This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding |
| − | + | site.</nowiki> | |
</li> | </li> | ||
</ol> | </ol> | ||
| Line 51: | Line 46: | ||
<ol class="ui ordered list"> | <ol class="ui ordered list"> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a | + | <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005:</a> This part is the promoter circuit of BBa_K2546002.</nowiki> |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a | + | <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006:</a> This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to |
| − | + | let three gene be expressed in one circuit.</nowiki> | |
| − | + | ||
</li> | </li> | ||
<li value="・"> | <li value="・"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a | + | <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012:</a> This part is the promoter circuit of BBa_K2546010.</nowiki> |
| − | + | ||
</li> | </li> | ||
</ol> | </ol> | ||
| Line 78: | Line 70: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 87: | Line 79: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 96: | Line 88: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 105: | Line 97: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 114: | Line 106: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 123: | Line 115: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 132: | Line 124: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 141: | Line 133: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a> |
</td> | </td> | ||
<td>Plasmid_Backbone </td> | <td>Plasmid_Backbone </td> | ||
| Line 150: | Line 142: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a> |
</td> | </td> | ||
<td>Plasmid_Backbone </td> | <td>Plasmid_Backbone </td> | ||
| Line 159: | Line 151: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009</a> |
</td> | </td> | ||
<td>Plasmid_Backbone </td> | <td>Plasmid_Backbone </td> | ||
| Line 168: | Line 160: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 177: | Line 169: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
| Line 186: | Line 178: | ||
<tr> | <tr> | ||
<td class="selectable"> | <td class="selectable"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012 </a> | + | <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a> |
</td> | </td> | ||
<td>Coding </td> | <td>Coding </td> | ||
Revision as of 18:56, 17 October 2018
Parts Overview
Parts
Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.
Basic Parts
- <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001:</a> This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds</nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004:</a> This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3. </nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007:</a> This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3. </nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008:</a> This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3. </nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010:</a> This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.</nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011:</a> This part is coding for Laccase, which is related to lignin degradation.</nowiki>
Intermediate Parts
- <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002:</a> This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.</nowiki>
Composite Parts
- <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005:</a> This part is the promoter circuit of BBa_K2546002.</nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006:</a> This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.</nowiki>
- <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012:</a> This part is the promoter circuit of BBa_K2546010.</nowiki>
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
<a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000</a> |
Coding | dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf | Tsu-Wang Sun | |
<a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a> |
Coding | 2-haloacid dehalogenase(HAD) | Tsu-Wang Sun | 780 |
<a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a> |
Coding | 2-haloacid dehalogenase plus laccase with robosome binding site | Tsu-Wang Sun | 2408 |
<a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003</a> |
Coding | p+HL | Tsu-Wang Sun | 6 |
<a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a> |
Coding | pME60C shuttle vector | Tsu-Wang Sun | 6489 |
<a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a> |
Coding | 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site | Tsu-Wang Sun | 2451 |
<a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a> |
Coding | TonB + 2-haloacid dehalogenase + laccase | Tsu-Wang Sun | 4721 |
<a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a> |
Plasmid_Backbone | cm 4657 | Tsu-Wang Sun | 770 |
<a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a> |
Plasmid_Backbone | pME60C2 shuttle vector | Tsu-Wang Sun | 5491 |
<a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009</a> |
Plasmid_Backbone | kan bbrf2 | Tsu-Wang Sun | 4218 |
<a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a> |
Coding | TonB-dependent receptor (TonB) | Tsu-Wang Sun | 2244 |
<a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a> |
Coding | Laccase (TLcc) | Tsu-Wang Sun | 1584 |
<a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a> |
Coding | TonB with promoter and ribosome binding site | Yu-Hsi Lin | 2305 |