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Revision as of 19:08, 17 October 2018
Parts Overview
Parts
Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.
Basic Parts
- BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
- BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
- BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
- BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
- BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
- BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.
Intermediate Parts
- BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.
Composite Parts
- BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
- BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
- BBa_K2546012: This part is the promoter circuit of BBa_K2546010.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2546000< | Coding | dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf | Tsu-Wang Sun | |
| BBa_K2546001< | Coding | 2-haloacid dehalogenase(HAD) | Tsu-Wang Sun | 780 |
| BBa_K2546002< | Coding | 2-haloacid dehalogenase plus laccase with robosome binding site | Tsu-Wang Sun | 2408 |
| BBa_K2546003< | Coding | p+HL | Tsu-Wang Sun | 6 |
| BBa_K2546004< | Coding | pME60C shuttle vector | Tsu-Wang Sun | 6489 |
| BBa_K2546005< | Coding | 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site | Tsu-Wang Sun | 2451 |
| BBa_K2546006< | Coding | TonB + 2-haloacid dehalogenase + laccase | Tsu-Wang Sun | 4721 |
| BBa_K2546007< | Plasmid_Backbone | cm 4657 | Tsu-Wang Sun | 770 |
| BBa_K2546008< | Plasmid_Backbone | pME60C2 shuttle vector | Tsu-Wang Sun | 5491 |
| BBa_K2546009< | Plasmid_Backbone | kan bbrf2 | Tsu-Wang Sun | 4218 |
| BBa_K2546010< | Coding | TonB-dependent receptor (TonB) | Tsu-Wang Sun | 2244 |
| BBa_K2546011< | Coding | Laccase (TLcc) | Tsu-Wang Sun | 1584 |
| BBa_K2546012< | Coding | TonB with promoter and ribosome binding site | Yu-Hsi Lin | 2305 |