Team:NCHU Taichung/Parts

NCHU_Taichung

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Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001</a>: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
  2. <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  3. <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007</a>: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  4. <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008</a>: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
  5. <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010</a>: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
  6. <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011</a>: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002</a>: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

  1. <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005</a>: This part is the promoter circuit of BBa_K2546002.
  2. <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006</a>: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
  3. <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012</a>: This part is the promoter circuit of BBa_K2546010.

<thead> </thead> <tbody></tbody>
Name Type Description Designer Length 
             <a href="http://parts.igem.org/Part:BBa_K2546000">BBa_K2546000 </a>
Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun 
             <a href="http://parts.igem.org/Part:BBa_K2546001">BBa_K2546001 </a>
Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780 
             <a href="http://parts.igem.org/Part:BBa_K2546002">BBa_K2546002 </a>
Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408 
             <a href="http://parts.igem.org/Part:BBa_K2546003">BBa_K2546003 </a>
Coding p+HL Tsu-Wang Sun 6 
             <a href="http://parts.igem.org/Part:BBa_K2546004">BBa_K2546004 </a>
Coding pME60C shuttle vector Tsu-Wang Sun 6489 
             <a href="http://parts.igem.org/Part:BBa_K2546005">BBa_K2546005 </a>
Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451 
             <a href="http://parts.igem.org/Part:BBa_K2546006">BBa_K2546006 </a>
Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721 
             <a href="http://parts.igem.org/Part:BBa_K2546007">BBa_K2546007 </a>
Plasmid_Backbone cm 4657 Tsu-Wang Sun 770 
             <a href="http://parts.igem.org/Part:BBa_K2546008">BBa_K2546008 </a>
Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491 
             <a href="http://parts.igem.org/Part:BBa_K2546009">BBa_K2546009 </a>
Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218 
             <a href="http://parts.igem.org/Part:BBa_K2546010">BBa_K2546010 </a>
Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244 
             <a href="http://parts.igem.org/Part:BBa_K2546011">BBa_K2546011 </a>
Coding Laccase (TLcc) Tsu-Wang Sun 1584 
             <a href="http://parts.igem.org/Part:BBa_K2546012">BBa_K2546012 </a>
Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305