Team:NCHU Taichung/Parts Improved
Engineered part: BBa_J364007 in pSB1C3 to become a shuttle vector ( BBa_K2546004, BBa_K2546007, BBa_K2546008)
See also "Result".
This part is an improvement of BBa_J364007 in pSB1C3. The aim of our project is to engineer endophyte for phytoremediation. To prove our gene work well, we use BBa_J364007, pBBR1, and pMP4657 to get shuttle vector. As phytoremediation become more popular, the broaden rang plant using plasmid become more important. A great shuttle vector let the function of gene work well in different genus bacteria. So, making a shuttle vector is not only for our project but also for other people. pSB1C3 is a common use plasmid. We do a large improve which is changing the Ori of pSB1C3. We ligate Ori from pBBRMCS, or pMP4657 and the rest of BBa_J364007. Thus, we obtain a vector having prefix and suffix that can provide cloning gene. We transform BBa_K2546004 in Burkholderia cenocepacia strain 869T2, and test the efficient of green fluorescence protein. By interlab protocol: Cell growth, sampling, and assay, we can analyze the BBa_K2546004 work.
The follow is the assay of BBa_J364007(pSB1C3) in Escherichia coli, BBa_K2546004-GFP in Escherichia coli, and BBa_K2546004-GFP in Burkholderia cenocepacia strain 869T2
The quantity of GFP/per OD performed by BBa_J364007 (pSB1C3) in Escherichia coli, BBa_K2546004-GFP in Escherichia coli, and BBa_K2546004-GFP in Burkholderia cenocepacia Strain 869T2.