Team:SUSTech Shenzhen/Parts
Gold Medal
For Improvement in Gold Medal, we have improved the original GFP part (BBa_E0040) with a TCF-TATA promoter, generating a perfect reporter system for WNT ligand in our project (BBa_K2591014). It works as an improved reporter that could report even at a weak expression levels with higher efficiency. Also, we have improved the BGH polyA part (BBa_K2217015) with two LoxP sites to make a switch by Cre (BBa_K2591013) (Find more details in our part page here). We provide those well characterized and validated improved parts to the iGEM community in the hope of a broad range of synthetic biology applications.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K2591013 | Regulatory | LoxP-BGH polyA-LoxP | 305 |
| BBa_K2591014 | Reporter | TCF-TATA-GFP | 978 |
Silver Medal
For Validated Part in Silver Medal, we added three parts (BBa_K2591010, BBa_K2591011, and BBa_K2591012), consisting of gRNA of genes together with scaffold, all of which were well characterized, validated, and used in our project. (Find more details in our part page here)
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K2591010 | RNA | gPor-scaffold | 173 |
| BBa_K2591011 | RNA | gWls-scaffold | 174 |
| BBa_K2591012 | RNA | gWnt3a-scaffold | 174 |
Bronze Medal
For Characterization in Bronze Medal, we have characterized two parts, BBa_I714031 and BBa_K1592002, and repaired single errors in their sequences by artificial mutagenesis, which may potentially influence their function.
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