Aachen

Region: Europe - Germany
Section: Overgraduate
Track: Diagnostics
Poster: Zone 5 - #302 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room304 - 4:45 PM - 5:15 PM

Melasense

We plan on developing a melatonin biosensor. Our approach for the biosensor is to genetically modify Saccharomyces cerevisiae by integrating a highly specific human melatonin receptor into the cells. Melatonin has a high membrane permeability which permits us to use the nuclear retinoid z receptor (RZR) which is directly regulating gene expression. We express the RZR as a fusion-protein with the recognition sequence of the human estrogen receptor alpha (ERα). When melatonin is bound, the modified receptor binds to the estrogen receptor responsive element (ERE) and as a consequence regulate expression of firefly luciferase reporter genes. In our second approach, we will use the membrane-receptor MT1 for our biosensor. When melatonin binds to the G protein-coupled receptor, β-arrestins can be recruited. This mechanism allows us to use an enzyme fragment complementation assay based on two fusion-proteins.

Aalto-Helsinki

Region: Europe - Finland
Section: Overgraduate
Track: New Application
Poster: Zone 4 - #229 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room304 - 4:45 PM - 5:15 PM

Silkolor - A sustainable approach to dyeing industry using fusion proteins

Textile dyeing is one of the biggest polluters of natural waters. Many of the synthetic dyes used are non-biodegradable, toxic and large amounts of them end up in waters during the dyeing process. Natural dyes, although less toxic than synthetic ones, require mordants in order to bind to the fabric. Mordants often contain aluminum or other metals, which are harmful to the environment. We are addressing the problem by using two types of colorful fusion proteins. Chromoproteins are fused with binding domains to create colorful proteins which can bind cellulose or keratin based materials, such as cotton or wool, respectively. Spider silk is added to some of the proteins in order to make colored silk proteins that can be made into fibers, which would erase the need for the dyeing step from the textile value chain completely. Our experiments were focused on binding tests and silk fiber production.

ACIBADEM ISTANBUL

Region: Asia - Turkey
Section: Undergraduate
Track: Therapeutics
Poster: Zone 5 - #305 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room310 - 11:30 AM - 12:00 PM

LTNF 2.0: Circularized Venom Neutralizing Factor

The Opossum (Didelphimorphia) is an animal with a very unique characteristic; it displays an outstanding resistance to toxins, snake venoms in particular. This anti-venom ability is gained through a single protein; the Lethal Toxin Neutralizing Factor (LTNF). We are attempting to produce an improved version of this anti-venom, LTNF 2.0 if you will, as a synthetic anti-venom for human use. LTNF 2.0 incorporates the post-translational modification process known as circularization, a process that comprises of adding cysteine amino acids to both ends of a polypeptide chain; triggering the formation of a disulphide bridge, ultimately leading to a circular structure, hence the name circularization. Circularized proteins are known for not only greater stability but also greater efficacy of the protein, thereby improving its shelf life and lowering the required dosage for treatment, ultimately providing a more efficient bioproduct.

AFCM-Egypt

Region: Africa - Egypt
Section: Overgraduate
Track: Therapeutics
Poster: Zone 2 - #153 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room311 - 2:15 PM - 2:45 PM

Microbiota: Opening Doors to New Horizons in Colorectal Cancer Therapy

Colorectal cancer (CRC) is considered one of the most common cancers and accounts for almost half a million deaths annually worldwide. Tremendous progress has been made in understanding the role of the immune system in driving the development of cancers, including CRC. As sensors of cell death and tissue remodeling, Toll like receptors(TLRs) may have a universal role in cancer. There are different TLRs that respond to a variety of Pathogen associated molecular pattern (PAMPs) such as bacterial lipopolysaccharide . The evidence of existence of relevance between bacterial microbiota and carcinogenesis is increasing. it's suggested that microRNAs act as ligands of TLRs playing a role in epigenetic immune modulation. In this study, We will assess the therapeutic efficacy of microbiome based approach as novel therapeutic strategy in restoring normal Toll lie receptor signaling in CRC cell line .

AHUT China

Region: Asia - China
Section: Undergraduate
Track: Environment
Poster: Zone 2 - #99 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room208 - 3:15 PM - 3:45 PM

Carbon dioxide purifier

With greenhouse effect becoming a widespread concern in recent years, how to effectively capture CO2 has become a worldwide problem. At present, CO2 capture mostly includes absorption, adsorption and membrane methods, etc., which have problems with high cost, high energy consumption for regeneration and secondary pollution. CO2 capture using carbonic anhydrase has attracted extensive attention due to its high catalytic efficiency and environmentally friendly properties. First, our project successfully expressed wide type carbonic anhydrase in E. coli, however, its industrial application was limited due to poor stability and easy inactivation. Therefore, based on this, molecular simulation technology was used to investigate effect of amino acid residues mutation on the conformation and activity of enzyme, and the mutant carbonic anhydrase with higher thermal stability was obtained. The experimental results showed that the purified mutant carbonic anhydrase exhibited higher stability and activity than wild type carbonic anhydrase, achieving efficient capture of CO2.

Aix-Marseille

Region: Europe - France
Section: Overgraduate
Track: Therapeutics
Poster: Zone 2 - #128 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room311 - 3:15 PM - 3:45 PM

Breaking bugs

An alternative weaponry must be found to replace the harmful and expensive traditional insecticides, that are now nearly useless against bed bugs. In fact, they developed multiple resistance mechanisms (exoskeleton thickening and enhanced metabolic pathways). The breaking bugs project aims to provide a human-friendly, and efficient solution to eliminate bed bugs. The plan is to elaborate an attractive lethal trap. We will use biosynthesized pheromones as a chemical lure to attract the bugs into the trap and infect them with Beauveria bassiana (an entomopathogenic fungus), causing a fatal epidemic. We produced several types of pheromones in E. coli and are running tests to create the optimal pheromone cocktail. We have worked on producing several enzymes and adding adjuvants to improve the killing efficiency and speed of the fungus. We had nationwide advertising of our project and obtained an indisputable validation from the public for our engagement in fighting bed bugs.

ASIJ Tokyo

Region: Asia - Japan
Section: High School
Track: High School
Poster: Zone 5 - #287 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room309 - 2:15 PM - 2:45 PM

A1AT deLIVERy - Using Stem Cell and CRISPR Technology to Combat Alpha-1 Antitrypsin Deficiency

Alpha-1 Antitrypsin deficiency is a common genetic disorder -- the defective gene for which is carried by 1 in 25 people -- which arises from a single base pair mutation in the SERPINA1 gene, resulting in the production of a form of antitrypsin prone to polymerization. The mutated antitrypsin then builds up in liver cells and is unable to inhibit proteases in the lungs, leading to damage in both. Using CRISPR-Cas9 technology, we aimed to fix the error in SERPINA1 so that proper antitrypsin can be produced. We will show proof of concept in E. Coli cells using osmy secretion tags and GFP as a reporter. We hope to design a liver organ bud using IPS cell technology to deliver function A1AT through collaboration with Dr. Kagimoto of Healios Japan KK.

ASTWS-China

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 2 - #152 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room302 - 11:30 AM - 12:00 PM

Environment-friendly Copper Ion Sense and Treatment System

With the continuous development of industrialization, the negative environmental effects caused by heavy metal pollution are becoming more and more significant. Owing to easy migration and difficult biodegradation, it poses more challenges to the treatment of heavy metals in the environment, especially in soil and water. In this study, we developed an engineered E. coli-based system to sensitively detect the copper concentration in industrial waste using synthetic biological methods. Meanwhile, we are trying to introduce a new gene to effectively capture copper ions in environment. If successful, this constructed biosystem could not only detect copper ions, but also enrich heavy metal pollutants (copper), form copper deposits and then purify the environment, which is portable, low-cost and environment-friendly.

Athens

Region: Europe - Greece
Section: Undergraduate
Track: Diagnostics
Poster: Zone 3 - #178 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room302 - 4:15 PM - 4:45 PM

GENOMERS: Toehold switch enabled viral detection via routine glucose monitoring technology

Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) is a virus with ~35% mortality rate, considered to be one of the most likely to cause major epidemics. We aim to develop a rapid, low-cost test for MERS-CoV for potential use in field diagnosis. Our biosensor is based on the toehold switch mechanism. The designed switches regulate the expression of trehalase, an enzyme which hydrolyzes the disaccharide trehalose to glucose. Thus, overall, the presence of viral load in the sample triggers glucose production, which is measured by a repurposed glucometer, signaling the diagnosis. Finally, attempting to accelerate the diagnosis, we lower the complexity of the switches using an alternative reporter, an engineered split trehalase. The two split fragments assemble to a functional enzyme through coiled-coil interactions. Our proposed diagnostic workflow is easily customizable for the detection of other viruses threatening global health, aiming to contribute to travel medicine and diagnostics.

Auckland MOD

Region: Asia - New Zealand
Section: Undergraduate
Track: Environment
Poster: Zone 5 - #316 - Thursday - Session A & B - 12:45 PM - 2:15 PM
Presentation: Thursday - Room312 - 12:00 PM - 12:30 PM

Improving the Farmer, Environment and Nitrogen Use Efficiency

Environmental pollution is a pressed global issue, even in clean, green New Zealand. Maintaining clean waterways is our responsibility as kaitiaki of the land (guardians in Te Reo Māori), but agricultural practices such as excess fertiliser application and cow effluent are flooding our New Zealand soils and waterways with urea. Taking a fluxomics approach in Arabidopsis thaliana, we are overexpressing a high-affinity urea transporter (DUR3) to upregulate the uptake of urea, and glutamine synthetase (GS1) to convert the toxic metabolite ammonia into glutamine. As a result, urea is removed more readily from the soil before it’s subject to groundwater leaching or surface run-off. We predict the increase in amino acid production will enhance plant growth. Applying our model to other plants like ryegrasses will allow farmers to grow pasture or forage crops that utilize urea on the paddock more efficiently, requiring less financial investment into urea fertilisers.

Austin LASA

Region: North America - United States
Section: High School
Track: High School
Poster: Zone 5 - #317 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room302 - 2:15 PM - 2:45 PM

Infection Detection: HIV1 Detection in Infants

HIV diagnosis of infants in the developing world continues to pose many problems as current diagnostic methods are inaccurate in infants and difficult to administer in the field. Recent research demonstrates CRISPR-associated enzyme Cas12a's ability to indiscriminately cleave ssDNA following recognition and cleavage of a dsDNA target strand, lending itself to nucleotide detection assays. Due to their high stability in cells, Cas enzymes such as Cas12a are prime candidates for lyophilized bacterial reagents ('cellular reagents') that can be stored at room temperature until resuspended for later use in the field. Our project aims to design an innovative HIV1 diagnostic system for infants that combines cellular reagents with a Cas12a assay. For the purposes of iGEM and biosafety, our team focused on demonstrating the following with purified enzymes and cellular reagents: isothermal amplification of viral DNA and detection of viral DNA by a Cas12a assay.

Austin UTexas

Region: North America - United States
Section: Undergraduate
Track: Foundational Advance
Poster: Zone 1 - #69 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room312 - 12:00 PM - 12:30 PM

A Broad Host Range Plasmid Kit for Engineering Non-Model Bacteria

Synthetic biologists often reach for a handful of well characterized organisms when designing experiments due to their ability to be reliably engineered with standard protocols. However, there are many non-model organisms that perform useful functions, survive extreme environments, or are optimized to produce certain materials which are largely ignored because the methods of engineering them are not well established. The broad host range kit aims to use genetic parts that function in a wide range of bacteria to make this process more efficient. The kit contains a combination of plasmid parts and assembled plasmids with origins of replication known to function in diverse bacteria. Each origin is linked to a fluorescent protein or chromoprotein so successful transformations can be easily identified when plated. Additionally, origins are associated with a specific barcode that can be sequenced to confirm the assembly. Several assemblies containing broad host range origins have been constructed.

Baltimore BioCrew

Region: North America - United States
Section: High School
Track: High School
Poster: Zone 4 - #237 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room304 - 2:45 PM - 3:15 PM

Coagulance Rx

In 2017, Baltimore suffered from 301 homicides due to gun violence. As students who live in Baltimore City, we knew that this issue needed to be addressed. We decided to create a cost-efficient alternative to current fibrinogen-laced bandages on the market. Our method to cause blood clots was by expressing Factor V activator RVV-V gamma in E.coli. We intend to embed this protease into a bandage to treat gunshot or stab victims. We have also worked to enhance the expression of tissue plasminogen activator (tPA), an enzyme that causes coagulated blood to degrade. We want to express an optimized sequence of tPA within E.coli. A purified form of this tPA would be used within an IV therapy for patients suffering from heart disease and other illnesses involving invasive blood clots. We hope to liberate communities within Baltimore by creating more balanced and equitable methods of treatment.

BCU

Region: Asia - China
Section: Undergraduate
Track: Environment
Poster: Zone 2 - #141 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room311 - 5:15 PM - 5:45 PM

Nicotine Degradation

Nicotine, a major alkaloid in tobacco plants, is a significant factor of evaluation for tobacco and cigarettes. Nicotine plays a critical role in smoking addiction and is well known to be harmful to human beings, because it easily crosses the blood-brain barrier and biological membranes. Meanwhile, with large quantities of tobacco products being produced and consumed, tobacco waste is entering the environment. So we want to find a key nicotine-degrading gene to degrade nicotine effectively in E.coli by synthetic biology. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 has been obtained by our team and a new expression vector has been constructed with promoter(J23119), ribosome binding site(RBS B0034), nicA ,terminator(B0015) in psb1c3. NICA expressed by E.coli top10 could catalyse and degrade Nicotine effectively from 35-50℃ and pH5 to pH8.

BFSUICC-China

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 2 - #95 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room309 - 2:15 PM - 2:45 PM

Biological toolkit for Copper detection

The world's production (supply) and consumption (demand) of copper have increased dramatically in the past 25 years. Massive mining and extensive using of copper results in serious contamination to environment, and then copper contamination threaten the balance of the whole ecosystem. We design a circuit that can better detect the amount of copper ions than before. We improved a previous Part by placing self-cleaving RNA ribozyme RioJ between the promoter of copA and RBS, and replacing reporter of GFP by sf GFP. PcopA is regulated by Cue R protein. We design a new part that is made up of L-arobinose inducing PBAD, RBS and Cue R coding sequence. Furthermore we connect the improved part and the new part together, which is the circuit of our project. It is found that Cue R protein of different concentration affects the response of Pcop A to Copper ions.

BGIC-Global

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 3 - #210 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room312 - 9:00 AM - 9:30 AM

Formaldehunter

In large cities of China, the population growth is accelerating. As a result, an increasing number of buildings are constructed and renovated, and problems of indoor air quality in newly decorated houses become more and more serious. Formaldehyde existing in paint and furniture may cause asthma, or even potentially leukemia. It is commonly acknowledged that formaldehyde volatile is very hard to control as it has long volatilization period. Unfortunately, current methods to remove formaldehyde are mostly either inefficient or expensive. Therefore, our project aims to develop an engineered E.coli to detect and eliminate the indoor formaldehyde safely and efficiently, when the concentration of which exceeds the legal limitation. By emitting florescence, the E.coli indicates the presence of formaldehyde and its effectiveness. Besides, the design of replaceable freeze-dried E.coli ensures the durability of the product. In this way, we would like to provide people with a real 'formaldehunter'.

BGU Israel

Region: Europe - Israel
Section: Overgraduate
Track: Therapeutics
Poster: Zone 4 - #250 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room309 - 9:30 AM - 10:00 AM

OriginALS - Prolong ALS Patients Surival

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that leads to a progressive muscle wasting and paralysis due to damage in motor neurons. However, no efficient treatment exists. The BGU-IGEM team aims to develop a system that will ultimately prolong survival of ALS patients by targeting microglia and reactive astrocytes, which are both non-neuronal cells that directly contribute to motor neuronal damage. Our approach is based on: (1) inhibition of toxic pro-inflammatory cytokines secretion in microglia cells and (2) on promoting intrinsic apoptotic signal in reactive astrocytes and preventing their toxic effect on motor neurons. Using modified genome editing technique, we build a system that specifically target toxic astrocytes and prevent the formation of new ones which hopefully will slow down the progression of the disease. As the reactivity of microglia and astrocytes is a common in neurodegenerative diseases, our novel approach could be expanded to other neurodegenerative diseases.

Bielefeld-CeBiTec

Region: Europe - Germany
Section: Overgraduate
Track: Environment
Poster: Zone 2 - #164 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room207 - 5:15 PM - 5:45 PM

nanoFactory: Recycling metal resources - Every particle matters!

Copper, silver and gold - metals are essential for our daily life but resources are dwindling. Industrial mining of metals and electronic waste cause pollution of the environment. Therefore, we established new approaches to recover valuable resources through synthetic biology. By enhancing bacterial abilities to scavenge metal ions from the environment we generated nanoparticles. We optimized Escherichia coli to accumulate metal ions as copper and iron by overexpression of dedicated importers and silencing of exporters while reducing the effects of oxidative stress. To gather nanoparticles from various metal ions we engineered the iron storage protein ferritin. Recycled into nanoparticles the metals could be used for various applications as demonstrated by printing electronics. Considering Dual Use aspects we decided to extract metal ions from pit water instead of dissolving electronics directly. Therefore, in close collaboration with leading experts we developed a customized cross-flow bioreactor for the mining industry.

Bilkent-UNAMBG

Region: Europe - Turkey
Section: Undergraduate
Track: Environment
Poster: Zone 3 - #173 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room309 - 10:00 AM - 10:30 AM

The Last Penicillin Binder

Water pollution originates from many contaminants and antibiotic waste is one of them. Antibiotics which remain in waste water after a treatment may cause bacteria to become multi resistant. In result of this, bacterial infections could spread rapidly and without having an efficient treatment. Current chemical methods of water purification require high cost and energy to be effective. To solve this problem with a cheaper method, our team modified bacteria to bind penicillin remains in waste water. The bacteria produce biofilms which on the surface has penicillin binding peptides attached to csgA proteins. We aim to target beta-lactam rings of the penicillin with these peptides. Our modified bacteria produces an iron-storage protein, bacterioferritin. Then using a magnetic field, we plan to pull away the penicillin-captured-bacteria to which we have added magnetic property with bacterioferritin proteins.

Bio Without Borders

Region: North America - United States
Section: Overgraduate
Track: Diagnostics
Poster: Zone 1 - #62 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room309 - 2:15 PM - 2:45 PM

Blueblood

Horseshoe crab (Limulus polyphemus) blood is the basis for the LAL clotting test for endotoxins in injectable formulations. Harvesting crabs for this purpose has endangered this 350 million-year-old species. The first step in the cascade that characterizes the LAL test is activation of the protease Factor C by contact with endotoxins. We have devised a replacement for the LAL test using cloned Factor C and an artificial substrate consisting of a reporter fused to cellulose binding domain with the Factor C protease site connecting them. The substrate is bound to paper by the cellulose binding domain. When exposed to Factor C mixed with the injectable liquid formulation to be tested, the presence of endotoxins will activate the protease and the substrate will be cleaved, releasing the reporter. Our aim is to develop the most cost-effective and simple device possible so that it can be used by everyone.

BioIQS-Barcelona

Region: Europe - Spain
Section: Overgraduate
Track: Diagnostics
Poster: Zone 2 - #106 - Thursday - Session A & B - 12:45 PM - 2:15 PM
Presentation: Thursday - Room208 - 9:30 AM - 10:00 AM

IN SITU PERSONALIZED DIAGNOSIS KIT FOR CELIAC DISEASE

In our iGEM Project we will design a personalized gluten sensor through a synthetic biology approach. To do so, we will build a model based on the HLA expression of the patient which will be coupled to a sensor, allowing the detection of reactive epitopes. Our sensor: a) Will be built according to the patient HLA, allowing the detection of specific reactive epitopes independently of the food source. b) Will be able to detect reactive epitopes even in fermented foods. c) The methodology implemented in our sensor could be used for the identification of new reactive epitopes and unknown allelic variants. d) Requires only a DNA sample of the patient. Therefore, the methodology and application of our sensor could be extended for the detection of other HLA related disorders as well as the generation of new research lines for the diagnosis, detection and basic knowledge of these type of disorders.

BioMarvel

Region: Asia - Korea
Section: High School
Track: High School
Poster: Zone 1 - #64 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room309 - 2:45 PM - 3:15 PM

Functional Fusion Protein-Based Biochip for Diagnosis and Monitoring of Heart Failure

The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure. DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies. An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip shown in this study could be used for diagnosis and monitoring of heart failure.

BIT

Region: Asia - China
Section: Undergraduate
Track: Diagnostics
Poster: Zone 5 - #271 - Thursday - Session A & B - 12:45 PM - 2:15 PM
Presentation: Thursday - Room309 - 12:00 PM - 12:30 PM

JACOB 2.0:Reborn for Optimization

Last year, our JACOB 1.0 used the competitive reaction between target protein and aptamer-complementary chain complexes to achieve signal conversion for sample markers' early detection. This year, we adopted the idea of JACOB, detect CKMB protein to monitor myocardial infarction. Then, in order to adapt to different needs of detection. We designed two sets of independent fluorescent expression systems that each has advantages. One is to modify the molecule SAM on the complementary chain, and to control engineering bacteria to produce GFP by using SAM-riboswitch. Another method is to combine the Spinach Probe with the complementary strand to form a stem loop structure to capture the Fluorescein (DFHBI) then produce fluorescence. We designed microfluidic chip that can carry the whole biological reaction process. We integrated the peristaltic pump on it also, so the chip and detection equipment are completely separated, which greatly reducing the volume of the overall instrument.

BIT-China

Region: Asia - China
Section: Undergraduate
Track: New Application
Poster: Zone 4 - #235 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room312 - 3:15 PM - 3:45 PM

Who can get an A?

Reactive oxygen species (ROS) is considered as the main reason of human aging through damaging DNA, attacking membrane and inducing apoptosis. Now many antioxidants adding in food, cosmetic and some medical production claim they can clear oxidative damages. Although many methods of measuring antioxidants capability are precise in vitro, there is no standard method for living cell. Therefore our project is to construct a system which can determine the activity of antioxidants in vivo. We chose Saccharomyces. cerevisiae as host and accumulated ROS by overexpressing genes. After reacting with antioxidants, the remaining ROS could reflect the antioxidant activity which could be detected by a redox sensor, roGFP2-Orp1. Additionally, a feedback gene circuit was set to avoid the overproduction of ROS which injured our yeast. Compared with the traditional methods, our system requires a milder environment, damage-free and with higher biologically relevant which make our system more reliable.

BJRS China

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 4 - #249 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room304 - 3:15 PM - 3:45 PM

OxygenMAX

Previous work have shown that the expression of bacterial Vitreoscilla hemoglobin (VHb) can help bacteria utilize oxygen more efficiently. However, the bacterial cell membrane makes efficient hemoglobin-oxygen contact a challenge. Based on this, our team designed a VHb surface display system to express VHb on the outermost shell of the bacteria to raise the hemoglobin-oxygen contacting efficiency. Consequently，this could help bacteria tolerate the low oxygen environment better. We named this system OxygenMAX system. Basically, our OxygenMAX system can be applied to industrial fermentation to raise the high-cell-density growth of the engineering bacteria in bioreactors. Also, allowing for the better growth ability of the OxygenMAX system carried bacteria, our system can help avoid contamination with miscellaneous bacteria in industrial fermentation. Moreover, our OxygenMAX system can be applied to other low-oxygen engineering bacteria working condition like biosensor in intestinal tract, water or soil.

BNDS CHINA

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 2 - #147 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room208 - 4:45 PM - 5:15 PM

A. hydrophila Killer

Aeromonas hydrophila is an ubiquitous gram-negative opportunistic pathogen in aquaculture. Every year, it causes a variety of diseases in fish. The symptoms include ulcers, fin rot, and hemorrhagic septicaemia. When A. hydrophila enters fish body, it often colonise in the gastrointestinal tract first. The pathogen's virulence factors secretion systems are controlled by N-acylhomoserine lactone (AHL)-dependent quorum-sensing system based on the ahyRI locus. Since the pathogen has developed resistance to most common antibiotics, our project targets to develop an A. hydrophila killer by engineering the fish probiotics, Escherichia coli MG1655. The killer expresses lactonase to degrade quorum sensing signals from the pathogen in aim of reducing the production of virulence factors. Also, it expresses antimicrobial peptides (AMPs) to inhibit the growth of A. hydrophila directly. We plan to regulate lactonase and AMPs expression by using Prhl promoter, which is induced by the pathogen's dominant quorum sensing molecule, C4-HSL.

BNU-China

Region: Asia - China
Section: Undergraduate
Track: Foundational Advance
Poster: Zone 3 - #208 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room302 - 5:15 PM - 5:45 PM

Screening Advantageous Mutants - a Self-enrichment System

Bioengineering uses stable, highly productive mutants, target strains, which contain foreign genes. However, screening these mutants costs vast time and workforce, and it is difficult to avoid using antibiotics. We applies synthetic biology methods, constructing a novel pathway to screen mutants by giving target strains growth advantages. Here, utilizing AND gate, the gene of interest expresses to a certain level, making the downstream pchAB gene express and catalyze the generation of salicylic acid（SA）. SA can activate the expression of glucose dehydrogenase, which gives the target strains an additional growth advantage. Besides, we integrate the most important control module of the system into the genome using Chemically Inducible Chromosomal Evolution(CIChE). In summary, the target strain will finally obtain the greatest growth advantage in bacterial suspensions and achieve screening internally. This new screening method is simple to operate and provides a new idea for antibiotic-free screening.

BOKU-Vienna

Region: Europe - Austria
Section: Overgraduate
Track: Information Processing
Poster: Zone 4 - #260 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room304 - 10:00 AM - 10:30 AM

ROBOCROP –Turning Genes ON and OFF, in Yeast and Arabidopsis through a dCas9 Toggle Switch

Our goal, communication with eukaryotes, is achieved through the heart piece of our model, the dCas9 Toggle Switch. This will allow switching between two stable states of gene expression. It consists of 2 gene classes which we simply call the ON and OFF genes. One gene in each class, which is considered the primary gene, codes for a gRNA which represses the antagonistic set of genes by binding to dCas9 and further blocking transcription though CRISPR Interference. The switch can be activated either by signal molecules binding to a receptor or directly by liposome bound gRNA that is taken up by the cell. As a proof of concept, the ON gene contains a GFP coding sequence as a reporter gene. Our design is very universal and has many possible applications in the lab and in agriculture, such as controlling flowering time of plants to protect them from late frost.

Bordeaux

Region: Europe - France
Section: Overgraduate
Track: Environment
Poster: Zone 5 - #277 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room302 - 5:15 PM - 5:45 PM

Far Waste in the Landes Forest

This year IGEM Bordeaux Team would like to find an alternative to an entire segment of the traditional petrobased chemistry by a new green biobased chemistry. Indeed, we would like to focus on the biocatalysis of the hydroxymethylfurfural (HMF) in 2,5-furandicarboxylic acid (FDCA). Don't worry, it is not as complicated as it appears. HMF is a by-product of the lignocellulosic biomass treatment. Its toxicity toward microorganisms leads to big issue for many companies which want to use these microorganisms to produce molecules of interest from lignocellulosic biomass. Our project consists in HMF detoxification by using it as a substrate to produce FDCA through bacteria .FDCA was identify as one of most promising biobased molecules which can replace many polymers such as PET (and other petrobased molecules). We suggest a sustainable alternative, eco-friendly and independent from fossil resource.

BostonU

Region: North America - United States
Section: Undergraduate
Track: Foundational Advance
Poster: Zone 2 - #160 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room310 - 9:00 AM - 9:30 AM

Characterizing Inducible Tools for Dynamic Control of Transcription in Budding Yeast

BostonU is characterizing and optimizing two light-inducible promoters, LOV2 and PhiReX, in S. cerevisiae to improve eukaryotic transcriptional control with applications in industrial fermentation. Light-inducible promoters lend greater spatiotemporal control over transcription than small molecule-inducible promoters. Further, LOV2 is activated by blue light and PhiReX by red light, allowing for multiplexed control. We characterize these systems using the eVOLVER, a novel automated cell culturing platform developed by Brandon Wong at Boston University's Khalil Lab. The eVOLVER's specificity and high throughput allows for unprecedented characterization across light pulse programs, temperature, and OD thresholds. We then apply LOV2 and PhiReX to the violacein pathway, demonstrating the induction of four distinctly-colored phenotypes in a single strain, providing a proof-of-concept for the multiplexed control and finely-tuned expression of genes required for effective control of metabolic flux via transcriptional regulation.

BostonU HW

Region: North America - United States
Section: Undergraduate
Track: Open
Poster: Zone 3 - #195 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room312 - 2:15 PM - 2:45 PM

TERRA: An application agnostic device that selectively dispenses the outputs of microfluidic chips

While microfluidics is not new to synthetic biology, they're not widely used by or accessible to many biologists. The current 'lab on a chip' microfluidic chips are highly specialized to each experiment and expensive to manufacture. In order to analyze the results of the experiments on microfluidic chips, many designs incorporate embedded sensors directly on chip. However, labs already have dedicated equipment to analyze experiments, such as plate readers and flow cytometers. Traditional analytical equipment could be used to analyze the outputs of microfluidic chips if the outputs were dispensed selectively into standard vessels, such as a 96-well plate. This would increase the design space for microfluidic experiments, enabling biologists to incorporate microfluidic chips into their workflows without having to fabricate highly specialized chips. To accomplish this we have created TERRA, an application-agnostic system that selectively dispenses the outputs from a microfluidic chip into standard vessels for downstream analysis.

Botchan Lab Tokyo

Region: Asia - Japan
Section: Undergraduate
Track: Food & Nutrition
Poster: Zone 1 - #27 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room207 - 10:00 AM - 10:30 AM

Bacterial Supplement ~Amino Acid Synthesis Model from Nitrogen in Intestinal Bacteria~

Among some kind of nutrients, proteins are very important elements for body formation. However, it is difficult for people in poor areas to continuously obtain protein rich foods. Therefore, in addition to these ingredients, we propose 'Bacterial Supplement' anyone can easily take it into the body. We got this idea from Papuans living in Papua New Guinea. Despite their low-protein diets, they have muscular bodies. It is thought that nitrogen fixing bacteria in their intestines are influencing on protein nutrition. We thought to construct a pathway to synthesize amino acids from nitrogen in E. coli, introducing it in the future. To synthesize amino acids, we first express nitrogenase to convert nitrogen to ammonia. We then express amino acid dehydrogenase to synthesize glutamate and phenylalanine from accumulated ammonia. We hope that our project will contribute to the solution of protein-energy malnutrition by fixing this E. coli in our intestinal flora.

British Columbia

Region: North America - Canada
Section: Undergraduate
Track: Manufacturing
Poster: Zone 2 - #121 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room312 - 12:00 PM - 12:30 PM

Co-Optimize: Distributed Metabolic Pathway of Naringenin and Kaempferol

Distributing metabolic pathways between microbial community members has shown significant potential for the large-scale production of complex, biologically-derived chemical products. Our goal is to address the challenge of regulating population dynamics in a synthetic microbial consortium, by improving the rate of production of naringenin and its pharmaceutically significant derivative, kaempferol, which has anti-cancer properties. This is done by distributing the synthesis of kaempferol between two E. coli strains and optimizing their relative proportions in co-culture. To optimize population dynamics for the production of kaempferol, we regulated the ratio of the two strains using GP2, a transcriptional inhibitor, under the control of a biosensor responsive to the pathway intermediate naringenin. This couples cell growth with the concentration of naringenin, allowing the co-culture to self-optimize based on pathway intermediate abundance. Using our system, we have demonstrated a novel way to optimize microbial polycultures for the synthesis of metabolically complex compounds.

BrockU

Region: North America - Canada
Section: Overgraduate
Track: New Application
Poster: Zone 2 - #89 - Thursday - Session A & B - 12:45 PM - 2:15 PM
Presentation: Thursday - Room306 - 11:00 AM - 11:30 AM

Lights, Camera, Flip!: Engineering a Light-Activatable Flip Recombinase for in vivo Genetic Manipulation

Flip recombinase is a versatile and important recombinase enzyme with broad applications in molecular genetic applications. Flip recombinase has been used to induce genetic mutations in vivo in numerous model organisms including bacteria, Drosophila, Zebrafish, and mouse and human cells. However, Flip recombinase activity is binary and thus cannot be precisely activated in time and space. Utilizing light sensitive protein interaction domains termed 'magnets', we have developed a light-sensitive optogenetic variant of Flip recombinase that can be controlled in Escherichia coli with exquisite spatio-temporal precision. We believe this Opto-Flip recombinase has the potential to be utilized in multiple model organisms, and will provide a novel tool allowing for precise molecular-genetic control for numerous future research and industrial applications.

BUCT-China

Region: Asia - China
Section: Undergraduate
Track: Environment
Poster: Zone 4 - #253 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room207 - 4:45 PM - 5:15 PM

Research and Construction of Fatty Acids and Glyoxylic Acid Operons

The regulation of expression in the process of life is the essence of life. The construction of gene expression regulation network has become the key to explain the mystery of life. However, due to its complexity and diversity, it is necessary to study its subunit ¬-operator first. . In this experiment, experiments were carried out by experimental ideas such as controlled experiments and deductive methods. Through the design process, operon prediction, operon verification, quantitative analysis, model establishment and other experimental processes, the research and construction of multi-class fatty acids and glyoxylate operons were carried out. Through many experiments, this experiment successfully constructed fatty acid, glyoxylate operon, and found a suitable substrate for fatty acid operons: hydroxy fatty acids. At the same time, quantitative experiments have also made some progress. Based on the qualitative and quantitative experiments, we also established the mode

Bulgaria

Region: Europe - Bulgaria
Section: Undergraduate
Track: Diagnostics
Poster: Zone 5 - #286 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room304 - 11:30 AM - 12:00 PM

The 65 CRISPRoses Story

We aim to create a CRISPR-based DNA diagnostics system that could be used for the detection of the most frequent mutations leading to cystic fibrosis. This genetic condition is considered to be the most common rare disease in Bulgaria. In most cases, the patient is initially misdiagnosed when sweat chloride level is used as an indicator. Our system relies on CRISPR's ability to recognize specific sequences. We plan on using different read-outs, our first idea being site-specific DNA cleavage if a cystic fibrosis associated mutation is present. Another approach would be a pair of dCas9 proteins, linked to split halves of a reporter molecule that restores its activity if the target sequence is identified. Not only could our system be applied in big healthcare facilities, but also in many small town hospitals, since it does not require expensive and sophisticated equipment, for instance DNA sequencing devices.

Calgary

Region: North America - Canada
Section: Undergraduate
Track: Foundational Advance
Poster: Zone 1 - #14 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room208 - 12:00 PM - 12:30 PM

Snip, Equip, Flip: Towards a Safer Gene Therapy

The ideal medicine is not a perfect treatment - it's a cure. Gene therapy, by correcting the genetic basis of disease, may represent humanity's best chance to develop such ultimate health solutions. Despite its unbounded potential, gene therapy is constrained by safety concerns surrounding existing gene transfer technologies. Highlighting a path forward, the 2018 Calgary team has developed a targeted gene integration strategy that leverages CRISPR knock-in, FLP recombinase vector integration and beta-resolvase backbone excision. Extending the integration strategy, the team tested chromatin-modifying elements to reduce variability in therapeutic gene regulation, built a droplet microfluidic device for a scalable gene transfer system, and developed a search tool to help iGEMers find past teams' software. As an extensible platform, the strategy promises greater reproducibility for transgenic research and industrial applications. As a vision for the future, the approach represents a shift away from legacy technologies and towards a safer gene therapy.

Cardiff Wales

Region: Europe - United Kingdom
Section: Undergraduate
Track: Environment
Poster: Zone 2 - #97 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room309 - 9:30 AM - 10:00 AM

RNAphid - an effective RNAi pesticide against Myzus persciae, expressed in Nicotiana benthamiana

Aphids are crop pests globally. They feed on a massive diversity of crops and can cause tremendous economic loss for farmers by reducing crop yields and grain sizes. They damage crops directly by feeding on plant vasculature, draining essential compounds, or indirectly, as hosts of a variety of plant viruses. Current agricultural practice is to use chemical pesticides, which are unfavourable due to off-target effects, harmfulness to humans, and developing resistance of aphids. Consequently, our team has attempted to produce an effective RNAi pesticide against Myzus persicae, the most economically detrimental aphid pest worldwide. In the vasculature of Nicotiana benthamiana, we express siRNAs that affect aphid bacteriocytes, cells that enable the survival of their essential symbiont, Buchnera aphidicola. We target genes BCR3 and SP3 to do this. Finally, we expand the limited PhytoBrick registry, with several plant promoters and reporter genes.

CCA-San Diego

Region: North America - United States
Section: High School
Track: High School
Poster: Zone 2 - #131 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room306 - 5:15 PM - 5:45 PM

HORIZON: Regulated Systems for Crude Oil Degradation, Coupled with Biohydrogen Production

Oil fuels our modern world, but unrefined oil contains carcinogenic compounds known as polycyclic aromatic hydrocarbons (PAHs). PAHs and Petrogenic PAHs can inflict lasting damage to entire ecosystems. Horizon harnesses the natural ability of microorganisms to degrade PAHs to catabolize them into nontoxic substances. Horizon then reuses the catabolic end products which can be metabolized by bacteria to produce clean energy by coupling the degradation pathways with sequences that upregulate hydrogen synthesis within E.Coli. Horizon also uses synthetic pathways to metabolize long n-chained hydrocarbons to fuel such hydrogen synthesis. These engineered E.Coli systems are then implemented in a bioreactor system optimized for bioremediation and capable of modulating between conditions for degradation and synthesis. To regulate the oil degradation and hydrogen synthesis pathways inexpensively, Horizon characterizes riboswitches and novel synthetic CRISPRi operators under riboswitch regulation. Ultimately, Horizon provides a comprehensive system for oil degradation and clean energy fuel production.

CCU Taiwan

Region: Asia - Taiwan
Section: Undergraduate
Track: New Application
Poster: Zone 1 - #45 - Friday - Session G & H - 6:45 PM - 8:15 PM
Presentation: Friday - Room311 - 2:45 PM - 3:15 PM

Liggreen

With the policy of restriction on plastic usage in Taiwan,we aim to produce a lignin-like polymer which can be applied as a lining for paper cups in place of plastic. We were inspired by the water resistant nature of lignin, but natural lignin has many weaknesses. By taking advantage of an oxidizing enzyme produced by engineered Pichia pastoris, we can bind monolignols together into a simpler polymer. This polymer, which we named Liggreen, is water resistant like plastic but decomposable and also heat resistant. Liggreen in paper cups is just one of many applications, so the future of Liggreen is prosperous.

CDHSU-CHINA

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 1 - #78 - Saturday - Session I & J - 12:45 PM - 2:15 PM
Presentation: Saturday - Room306 - 10:00 AM - 10:30 AM

Use genetically modified lactic acid bacteria to compound miraculin

Nowadays, it is nearly impossible for the patients with diabetes mellitus to enjoy the sweat foods, and our project is designed to solve that problem. So far, there is one thing that could help us reach our purpose-- Synsepalum dulcificum. The key is that the Miraculin in the Synsepalum dulcificum Could turn the taste of sour foods to sweat briefly, allowing patients with diabetes mellitus to enjoy the sweat taste. However, the current technology couldn't make the collection of Miraculin from the Synsepalum dulcificum easy and efficiently. Our goal is to compound the Synsepalum dulcificum protein by using genetically modified technology, and we believe that the new compound method could increase the quantity of the Miraculin and decrease the cost of production, with the intention to help diabetes mellitus patients.

Chalmers-Gothenburg

Region: Europe - Sweden
Section: Overgraduate
Track: Therapeutics
Poster: Zone 1 - #49 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room302 - 11:00 AM - 11:30 AM

DiYEASTive: Probiotic yeast for diagnosis and treatment of colorectal cancer

Our project uses Synthetic Biology to treat colorectal cancer. Our envisioned product is a pill containing genetically engineered probiotic yeast. The pill is ingested by the patient and passes through the digestive tract. If the ingested yeast cells encounter cancer cells in the colon, they will selectively attach to them. As more yeast cells accumulate, the secretion of anti-cancer molecules will be triggered. The yeast cells continuously produce gas vesicles which will refract ultrasound waves. This allows detection and monitoring using simple ultrasound imaging technology in an otherwise invisible and inaccessible part of the body. Meanwhile, the anti-cancer molecules specifically target and kill the cancerous cells, treating the patient with highly limited collateral damage.

CIEI-BJ

Region: Asia - China
Section: High School
Track: High School
Poster: Zone 1 - #42 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room312 - 4:45 PM - 5:15 PM

A yeast system for detection and degradation of aflatoxin B1

Our project is inspired by the possible contamination of the carcinogenic aflatoxins (AFTs), in Pu?er, a Chinese traditional fermented tea. We aim to design a genetically engineered yeast system to detect and degrade its widely occurred species AFT-B1. Our system contains three modules-induction, detection and degradation. The induction module was designed based on an iGEM project in 2017 using two fragments of an antibody against AFT-B1. The detection module utilizes enhanced yellow fluorescent protein to indicate the presence of ATF-B1. In the degradation module, four candidate enzymes were incorporated individually and their activities were assessed. Both detection and degradation modules are triggered when AFT-B1 bridges the two antibody fragments. Our design not only provides a parallel detection and degradation in yeast with potential practical value for Pu?er Tea and other agricultural products, but also establishes a convenient screening system for identifying novel AFT-B1-degrading enzymes.

Claremont

Region: North America - United States
Section: Undergraduate
Track: Environment
Poster: Zone 2 - #93 - Saturday - Session K & L - 6:45 PM - 8:15 PM
Presentation: Saturday - Room310 - 2:15 PM - 2:45 PM

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CMUQ

Region: Asia - Qatar
Section: Undergraduate
Track: Diagnostics
Poster: Zone 2 - #96 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room302 - 9:00 AM - 9:30 AM

Cas12a - Recognizing Carriers of recessive traits to save generations

Our approach overcomes the limitations of sequencing, it being a cost-ineffective, labour-intensive, and location-specific method. Utilizing CRISPR for purposes other than gene editing has allowed us to create a novel, field-ready, diagnostic technique for carriers of recessive traits. Cas12a proteins are DNA targeting enzymes that recognize DNA based on a guide RNA sequence designed to match a target. The binding initiates non-specific single-stranded DNA (ssDNA) cleavage activity in Cas12a sufficient to degrade linear and circular ssDNA within minutes. Through this, ssDNA attached to fluorescent dye and quencher, serving as reporters, will undergo degradation. Upon cleavage, the quencher is released and fluorescence is emitted. We designed, built and programmed a hand-held device that can detect the fluorescence with high sensitivity. Simply, DNA obtained from cheek swabs, inserted into the device, diagnoses carriers of Sickle Cell Anemia.

CO Mines

Region: North America - United States
Section: Undergraduate
Track: Environment
Poster: Zone 2 - #108 - Thursday - Session A & B - 12:45 PM - 2:15 PM
Presentation: Thursday - Room304 - 11:00 AM - 11:30 AM

Molecular Mining of Cadmium: Detecting and Binding Cadmium for Bioremediation

Heavy metal contamination at current and former mining sites is a significant environmental and human health problem. Cadmium (Cd) is one of the more commonly found metal contaminants and due to the highly toxic nature, even minute amounts can cause loss of function of the kidney and liver and loss of bone. We developed a rapid and efficient cadmium sensing and binding system that is capable of detecting cadmium down to 10 μM concentrations. When exposed to a minimum concentration of Cd, the cell expresses the green fluorescent protein (GFP). After Cd is detected, a metallothionein protein binds it and sequesters it in the periplasmic space in the E. coli cell. We will present data characterizing the performance of this system. The engineered system can be used for remediation efforts to remove Cd from the environment and process it safely.

ColegioFDR Peru

Region: Latin America - Peru
Section: High School
Track: High School
Poster: Zone 4 - #244 - Thursday - Session C & D - 6:45 PM - 8:15 PM
Presentation: Thursday - Room309 - 3:15 PM - 3:45 PM

Fishing for Mercury: Detecting and Removing Hg from Fish Meal.

Contamination of heavy metals is intoxicating the food chain at an alarming rate. We are working with T.A.S.A., exporter of anchovy fish meal to detect, accumulate, and isolate mercury (Hg) from their fish meal product. Our first construct contains a Hg accumulator and Green Fluorescence protein (GFP) to detect and accumulate the Hg. The second construct, with delayed expressed of a Killer Red (KR) protein, will kill the bacteria in response to light. We aim to characterize the delayed expression of the KR protein under three different RBSs using unique constructs. The construct enabling delayed expression of the KR protein will be coupled with GFP/accumulator construct. We are building the GFP/accumulator construct using overlapping PCR. Finally, we are designing and creating a container optimizing the efficiency of detection and removal of Hg from fish meal.

ColumbiaNYC

Region: North America - United States
Section: Undergraduate
Track: Diagnostics
Poster: Zone 4 - #240 - Friday - Session E & F - 12:45 PM - 2:15 PM
Presentation: Friday - Room302 - 9:30 AM - 10:00 AM